ISOLATION AND CHARACTERISATION OF LYMPHOCYTES Lab Report

ISOLATION AND CHARACTERISATION OF LYMPHOCYTES - Lab Report Example This is because this results in an inaccurate count. The same is true if the cover slip is moved after the sample is loaded. To make randomized counting only the 4 squares at each end were picked and then the 16 small squares within each large square, that is, in an organized zigzag along the rows. To make the counting standardized only cells lying on the bottom and right hand boundary were counted in and a cell lying on the top or left hand boundary were not counted in. This was in order to avoid counting cells twice. Normally, at least two squares should be counted, comprising more than 100 cells within each central counting area of every square. For higher accuracy, additional cells can be counted and the mean used to compute cell concentration. The result of cells counts were in the table 1 below. The other cells in the splenocyte preparation are red cells 3.82%, Granulocytes 6.1% as shown in the table 4 above. The abundance of small lymphocytes shows that the cell suspension has more immature cells which are maturing in the spleen to become large lymphocytes and T-lymphocytes. With time the population of small lymphocytes decreases as they develop to large lymphocytes, medium lymphocytes and T-lymphocytes. The spleen is made up of immune lymphocytes and that why in this experiment there were low number of other types of cells. Time was very important in this lab because long exposure could lead to lack of the reliability. This is because the population of splenocytes keep on changing since some cell has not matured fully and other are multiplying. For this lab the time limit was hour since In this lab exercise, Nigrosin was used to stain any dead cells. Nigrosin is an acidic stain. This means the stain readily gives up a hydrogen ion and becomes negatively charged. Since the surface of dead cells is negatively charged, the cell surface repels the stain. Thus slide glass will stain, but the dead cell will not. The dead

Article review Essay Example | Topics and Well Written Essays - 500 words - 2

Article review - Essay Example For example: In motivation and self-directed learning; the author stated that allowing the students to read instructions or to decide their preferred learning strategy could trains them to be self-directed learners as it increases student’s motivation, sense of competence, reading comprehension, and strategy use. Article III – The literature part is comprehensive since it provides general information such as the races that migrates to Portugal in the past up to now; the national institutional regulations on the academic achievements of migrant children; etc. Article III – Quantitative survey questionnaires were used to gather data required to test the correlation among the variables related to socio-economic status, nationality, and the history of their family migration with school performance. The authors adopted Portes and Rumbaut’s (2001) questionnaire design. Article III – A total of 1,843 respondents participated in the survey. Respondents are composed of 53% Portuguese children, 44.3% immigrant children, and 1.9% emigrants. Target respondents are students between the age bracket of 14 to 24 including few students who are about to turn 14 years old. The authors did not mention whether they have randomly selected the respondents from the state secondary schools in Oeiras – a municipal located in the Greater Lisbon Metropolitan Area in Portugal. Article III – Aside from testing the variables related to socio-economic status, nationality, and the history of their family migration with school performance, the researchers used two-fold category for children born in Portugal of first generation immigrant parents; and those born abroad with the exception that those who were born abroad and came to Portugal before the age of six were included in the first category. The purpose of categorizing the respondents is to test whether migration is a factor for